Pancreas and liver samples were vigorously homogenized with 2.five mL of MeOH/water/HCl employing an IKA T10 Ultra-Turrax at 24000 rpm for 1 min and after that exposed to ultrasound for 10 min. The mixture was centrifuged at 2880 g for five min. The methanolic phase was evaporated inside a SpeedVac concentrator equipped with a refrigerated vapor trap RVT4104 plus a vacuum pump v-710, then diluted