mosphere at 37uC. charged glass slides. All immunostaining procedures had been performed immediately after the sections were baked dry, de-waxed using xylene substitute and rehydrated within a graded ethanol series. Testis sections had been washed with PBS for five min and heated at 90uC for five min in pre-heated citrate buffer. The slides had been then incubated with rabbit anti-GPER Ab in PBS,