1
(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was determined by RT CR using Ex4-F and Ex6-R primer pair that amplifies the two Ex4a(+)WT1 and significant WT1 isoforms in two different WT1-expressing cancer cells (LU99B and K562). (B-D) Actin is utilised as an internal command. Benefits are representative of 3 unbiased experiments.(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was resolute